Professor, Dept. of Biochemistry & Molecular Biology, University of
Oklahoma Heath Sciences Center.
Harvard, BS 1984; University of CA, Irvine, PhD 1990.
Glycobiology researcher since 1981 in the fields of carbohydrate/protein
interaction and polysaccharide biosynthesis using the experimental tools
of molecular biology, enzyme biochemistry, and carbohydrate analysis.
Identified and molecularly cloned several bacterial and viral polysaccharide
Dr. DeAngelis has pursued glycobiology, the study of carbohydrates in
life processes, from his undergraduate years to the present time as
a Professor and researcher at the University of Oklahoma
Health Sciences Center. Several current projects in the DeAngelis laboratory
focus on essential polysaccharides found in the human body. The DeAngelis
laboratory has identified and/or molecularly cloned several HA hyaluronan
biosynthetic enzymes (synthases) from bacteria, vertebrates, and viruses.
Active research includes the pursuit of the structure/function relationships
of the synthases. His group is also cloning and manipulating the enzymes
involved in the synthesis of various polysaccharides that are very similar
or identical to the major members of the mucopolysaccharide family:
hyaluronan, heparin and chondroitin. The DeAngelis laboratory discovered the first chondroitin synthase from any source.
His overall basic science goals are to understand the fundamental mechanisms
of naturally occurring enzymes. As a result of this work, he has found
certain modules and motifs are responsible for specific sugar transfer
reactions. This knowledge has allowed him to pursue endeavors with potential
commercial applications. Some of his biotechnological research is focused
on (a) the design of novel polysaccharide and oligosaccharide molecules
for medical or industrial applications, and (b) the formation of biocompatible
coatings and surfaces for use in sensors or other implanted devices.
Selected Recent Publications:
DeAngelis, P.L. and Padgett-McCue, A.J. 2000: Identification and Molecular
Cloning of a Chondroitin Synthase from Pasteurella multocida Type F.
J. Biol. Chem. 275:24124-24129.
Jing, W. and DeAngelis, P.L. 2000: Dissection of the Two Tranferase
Activities of the Pasteurella multocida Hyaluronan Synthase: Two Active
Sites Exist in One Polypeptide. Glycobiology. 10:883-889.
DeAngelis, P.L. 1999: Molecular Directionality of Polysaccharide Polymerization
by the Pasteurella multocida Hyaluronan synthase. J. Biol. Chem. 274:26557-26562.
DeAngelis, P.L., Jing, W., Drake, R.R., and Achyuthan, A.M. 1998: Identification
and Molecular Cloning of a Unique Hyaluronan Synthase from Pasteurella
multocida. J. Biol. Chem. 273:8454-8458.
Pummill, P.E., Achyuthan, A.M., and DeAngelis, P.L. 1998: Enzymological
Characterization of Recombinant Xenopus DG42, a Vertebrate Hyaluronan
Synthase. J. Biol. Chem. 273:4976-4981.
DeAngelis, P.L., Jing, W., Graves, M.V., Burbank, D.E., and Van Etten,
J.L. 1997: Hyaluronan Synthase of Chlorella Virus PBCV-1. Science. 278:1800-1803.
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